The challenges with the genetics of the soybean cyst nematode kept scientists from assembling the full genome for years. The researchers sequenced the genome first by sequencing smaller portions and then piecing those portions together into the full genome. About a third of the nematode's 29,769 genes are repetitive, which complicated the sequencing and assembly process, said Rick Masonbrink, an associate scientist in the ISU Office of Biotechnology and lead author of the study.
Andrew Severin, co-author of the study and manager of the ISU Genome Informatics Facility, likened the genome to a jigsaw puzzle of a blue sky in which all the pieces are identical in shape and color. But long-read sequencing technology made it possible to assemble a high-quality genome.
For more details, read the ISU news release.