RNA interference (RNAi) is commonly used in plants to silence genes and study gene functions or improve crops. However, methods for fine-tuning the degree of induced silencing are missing. Thus, Lucio López-Dolz and the team came up with two techniques based on artificial small RNAs (sRNAs) to fine-tune RNAi efficacy in plants.
The first method entails modification of the precursor position from which the syn-tasiRNA is expressed. Using this method, it was shown that the accumulation and efficacy of Arabidopsis TAS1c-based syn-tasiRNAs progressively decreased as the syn-tasiRNA is expressed from positions more distal to the trigger miR173 target site. The second method tweaks the degree of base-pairing between the 3′ end of the syn-tasiRNA and the 5′ end of the target RNA.
Both methods have been successfully applied in Arabidopsis thaliana and Nicotiana benthamiana.
Read more results in Nucleic Acid Research.