Breeding technologies, whether conventional or modern, have been often used to enhance crop production. However, these breeding methods are sometimes laborious and complicated, especially when attempting to improve desired traits without inducing pleiotropic effects.
Targeted genome editing (TGE) technology using engineered nucleases, including meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) has been used to improve the traits of economically important plants.
These TGEs has emerged as novel plant-breeding tools that are alternative approaches to conventional breeding, but with higher efficiency.
Saminathan Subburaj of the Chungnam National University in South Korea, together with researchers from various academic institutions, described the basic principles of TGE as well as their advantages and disadvantages. Their study also discussed TGEs' potential use to improve the traits of horticultural crops.
For more information, read the article in Horticulture, Environment, and Biotechnology.